Molecular Analysis Techniques

General

• Code: 276-190704
• Semester: 7th
• Study Level: Undergraduate
• Course type: Special Background
• Teaching and exams language: Greek
• The course is offered to Erasmus students
• Teaching Methods (Hours/Week): Lectures (2) / Laboratory (2)
• ECTS Units: 5
• Prerequisite courses: (276-190105) Biology – Genetics
• Course homepage: https://exams-geo.the.ihu.gr/course/view.php?id=202
• Instructors: Imsiridou Anastasia

Course Contents

• Genetic Engineering-Biotechnology.
• Structure and function of nucleic acids.
• Central dogma of Molecular Biology.
• DNA denaturation – renaturation.
• Fine structure of the gene, biological definition of the gene.
• Gene regulation in prokaryotes and eukaryotes.
• Restriction endonucleases, formation and cloning of recombinant DNA, cloning vectors.
• Genetic modification techniques. Genetic modification in plants, genetic modification in fish, genetically modified products and the European Union, Bioethics.
• DNA libraries.
• Genetic identification lab equipment.
• DNA extraction.
• Agarose gel electrophoresis.
• Polymerase Chain Reaction (PCR).
• Restriction Fragment Length Polymorphism (RFLPs) analysis.
• Sequencing analysis.
• Random Amplified Polymorphic DNA (RAPDs) analysis.
• Real Time PCR.
• Variable Number of Tandem repeats (VNTRs) analysis.
• Allozyme analysis.

Educational Goals

The aim of the specific course is a first familiarization of the students with the Science of Genetic engineering and Biotechnology, as well as with the applications of these Sciences in various sectors. Students will also be taught the new genetic methodologies used today in the genetic analysis, as DNA extraction, agarose gel electrophoresis, PCR and sequencing analysis. Finally, they will learn how to use the results of their experiments as well as bioinformatics tools.

General Skills

• Work in an interdisciplinary environment.
• Generation of new research ideas.
• Promote free, creative and inductive thinking.
• Search, analyze and synthesize data and information using the necessary technologies.
• Independent work.

Teaching Methods

• Face to face.

Use of ICT means

• Teaching with Power-Point presentations.

Teaching Organization

Students Evaluation

• Writing exams at the end of the semester.

Recommended Bibliography

1. Ιμσιρίδου Θ. Αναστασία (2018). Τεχνικές Γενετικής Ανάλυσης – Εφαρμογές στα Τρόφιμα. Εκδόσεις “σοφία”. Θεσσαλονίκη.
2. Babiuk, L., Phillips, J. & Moo-Young, M. (1989). Animal Biotechnology. Pergamon Press Oxford.
3. Barnum, S. R. (1998). Biotechnology: An Introduction. Wadsworth Publishing Company. Belmont, CA.
4. Beaumont, A. R. & Hoare, K. (2003). Biotechnology and Genetics in Fisheries and Aquaculture. Blackwell Science.
5. Dillon, J.,  Nasim, A. & Nestmann, E. (1985). Recombinant DNA. Methodology. J. Wiley & Sons. N. York.
6. Holland, A. & Johnson, A. (1998). Animal Biotechnology and Ethics. Springer.
7. Lou, B., (1988). The recombination of genetic material. Academic Press Inc.
8. Mustafa, S., (1999). Genetics in sustainable fisheries management. Fishing News   Books. A division of Blackwell Science Ltd.
9. Rollin, B. E. (1995). The Frankenstein syndrome. Ethical and social issues in the genetic engineering of Animals. Cambridge University Press.
10. Russell P.J. (2009). iGenetics: A Molecular Approach (3rd Edition). Pearson Education Inc. – Benjamin Cummings. Boston, New York.
11. Sambrook, J. (2001). Molecular cloning. CSHL Press.
12. Smith, G. P. (1993). Bioethics and the Low.

Related Research Journals

1. Journal of Heredity.
2. Aquaculture Research.
3. Mediterranean Marine Science.
4. Food Control.
5. Journal of Nutrition, Food and Lipid Science.